Quick revision of chromatography for JEE/NEET
- Chromatography is extensively utilized for compound separation, purification, and identification purposes. - According to IUPAC, chromatography is a physical separation method involving two phases: one stationary and one mobile, with components distributed between them. - The stationary phase typically exists as a packed column (column chromatography) or other forms like flat sheets or thin layers adhering to backing materials such as glass (thin-layer chromatography). - In column chromatography, the mobile phase flows through the packed column, while in thin layer chromatography, it moves via capillary action. - Thin film stationary phases can be either liquid or solid, while the mobile phase can be liquid or gas, resulting in various chromatographic techniques. - Partition chromatography involves a thin film of liquid stationary phase adsorbed on an inert support, with the mobile phase being liquid or gas. - Paper chromatography, an example of partition chromatography, utilizes li